Abberior STED superresolution microscope


Partner lab price : -- €/hour
Public and private lab price: -- €/hour
Associated technologies:
  • 2D imaging
  • 3D imaging
  • High resolution

    • Brief description:
    Inverted microscope, fully motorized, controlled by Imspector software (Abberior). This microscope allows super resolution imaging in 2D STED or 3D STED, up to 3 colors.
    Excitation LASERS 405, 485, 561 and 640nm.
    STED LASERS 595nm & 775nm.
    Objectives :
    100X Plan SuperApochromat 1.4 Oil
    100X Plan SuperApochromat 1.35 Sil
    60X Plan Apochromat 1.2 W
    20X Plan Apochromat 0.75

    Detectors :
    4 Avalanche Photo Diodes & spectral detection.

    Autofocus & Z-drift compensation

    RESCue imaging mode in confocal & STED

    L1 environement

    For more information, contact us : This email address is being protected from spambots. You need JavaScript enabled to view it.
    2D & 3D STED

    Incubation :
    Stage Top Incubator : Fully controlled environment including temperature, pH (CO2) & hygrometry. Objective heater.

    Posted in Videomicroscopy / Super-resolution

    About MRI-CIA

    ;The MRI Center for Image Analysis (MRI-CIA)

    MRI-CIA has been created to answer the bioimage analysis needs of the users of MRI. We offer comprehensive support for bioimage analysis, from the start of a research project to publication. The support we provide is tailored to each individual case and can include one-on-one software training or the creation of custom analysis tools. We handle various aspects of image analysis, such as for example (list non-comprehensive):

    • image data handling
    • image restoration and registration
    • quantitative image analysis and visualization
    • instance segmentation and tracking
    • morphological and intensity quantification
    • colocalization analysis and spatial statistics

    MRI-CIA closely collaborates with the other engineers of the MRI core facility in order to ensure optimal image acquisition modalities for downstream quantitative analysis.

    Contact: This email address is being protected from spambots. You need JavaScript enabled to view it.

    Please find more information here: The MRI image analysis service.pdf
    Download a project project form: mri-cia-project-form.pdf

    STAFF

    volker2
    Head of MRI-CIA
    clement
    Bio-image analyst
    thibault
    Trainee M2

    Posted in English

    ARIA IIu Becton Dickinson

    
    Partner lab price : 48.99 €/hour
    Public and private lab price: 131.03 €/hour
    Brief description:
    Analyzer and cell sorter by flow cytometry - Becton Dickinson
    4 lasers, 2 physical parameters and 17 fluorescences

    Controlled by the FACSDiVa software (Becton Dickinson)
    Blue laser excitation- 488nm :
    Filter 1 (505LP - 530/30BP) : FITC, AF488, GFP, CFSE, BB515
    Filter 2 (550LP - 575/25BP) : PE
    Filter 3 (600LP - 610/20BP) : mCherry, PI, PE-TexasRed, PE-CF594
    Filter 4 (635LP - 660/20BP) : PE-Cy5, 7-AAD
    Filter 5 (685LP - 710/50BP) : PerCP, PerCP-Cy5.5, PerCP-eF710
    Filter 6 (755LP - 780/60BP) : PE-Cy7
    Violet laser excitation - 405nm :
    Filter 10 (450/50BP) : BD Horizon V450, Pacific Blue, AF405, BV421
    Filter 11 (505LP - 525/50BP) : BD Horizon V500, Pacific Orange, AmCyan, Qdot525, BV510
    Filter 12 (595LP - 610/20BP) : Qdot605, BV605, eF605NC, eF625NC
    Filter 13 (630LP - 670/30BP) : Qdot655, BV650, eF650NC
    Filter 14 (685LP - 710/50BP) : Qdot700, BV711
    Filter 15 (750LP - 780/60BP) : Qdot800, BV785
    Red laser excitation - 633nm :
    Filter 7 (660/20BP) : APC, AF647
    Filter 8 (690LP - 730/45BP) : AF700, APC-R700
    Filter 9 (755LP - 780/60BP) : APC-Cy7, eFluor780, AAF

    UV laser Excitation - 355nm :
    Filter 16 (450/50BP) : DAPI, Hoechst, Hoechst 33258, Hoechst Blue, Indo-1 (violet)
    Filter 17 (505LP - 530/30BP) : Hoechst Red, Indo-1 (blue)
    High speed cell sorting (up to 25000 events/sec) on 2 or 4 ways, ACDU (automatic cell deposition unit) for plates (6, 12, 24, 96 or 384 wells) or slides.
    Sterile sorts with possibility to expand the sorted cells in culture
    Intracellular or cell-surface staining, fixed or alive cells.

    L2 laboratory completely equiped : hood (subject to booking), centrifuge, pipets
    Ideal operating temperatures between 19 and 26°C

    Posted in 19 parameters sorter

    Becton Dickinson FACS Melody

    
    Tarif établissement partenaire: 25.68 €/heure
    Tarif établissement public et privé : 50.75 €/heure
    Brève description :
    Analyzer and cell sorter by flow cytometry - Becton Dickinson with 3 lasers, 2 physical parameters and 8 fluorescences.
    The system does not require the presence of a dedicated engineer, after training each user can use it alone.
    Excitation and detection :

    Excitation laser 405 nm:
    Filter 1 : 428/45 (DAPI, Hoechst)
    Filter 2 : 528/45 (CFP) or 755LP (BV)

    Excitation laser 488 nm :
    Filter 3 : 527/32, 507 LP (FITC, GFP, AlexaFluor 488...)
    Filter 4 : 700/54, 665 LP (PerCP, PerCP-Cy5.5, 7AAD...)
    Cell sorting on two ways for 1.5, 2 or 5 mL tubes.
    Cloning for plates 6, 24, 48, 96, 96 PCR or 384 wells or slides.

    Temperature control for input sample.
    Excitation laser 561 nm :
    Filter 5 : 582/15, (PE, DsRed...)
    Filter 6 : 613/18, 605 LP (mCherry, PE-CF594, PE-Texas Red, PI...)
    Filter 7 : 697/58, 665 LP (PE-Cy5, PE-Cy5.5...)
    Filter 8 : 783/56, 752 LP (PE-Cy7...)
    Inquiries may be sent to This email address is being protected from spambots. You need JavaScript enabled to view it.
    

    Posted in 8 parameters sorter

    CANTO II Becton Dickinson

    
    Partner lab price : 21.78 €/hour
    Public and private lab price: 59.44 €/hour
    Brief description:
    FACS Canto 2 Becton Dickinson cytometer
    3 Lasers, 2 morphological parameters and 8 fluorescences
    30 tubes carroussel
    Diva Software
    BLUE LASER 488nm

    Filter 1 (530/30) : FITC, EGFP, CFSE, TO-PRO-1, Alexa 488
    Filter 2 (585/42) : PE, SNARF, DyeCycle Orange, Alexa 546, DiOC
    Filter 3 (670LP) : PE-Cy5.5, PerCP-Cy5.5, Tri-Color, 7AAD, Alexa 700
    Filter 4 (780/60) : PE-Cy7, Alexa 750
    RED LASER 638 nm

    Filter 5 (660/20) : APC, Alexa647, Cy5, TO-PRO-3, SYTOX Red
    Filter 6 (780/60) : APC-Cy7, APC-H7, APC Alexa 750, APC eFluor780
    VIOLET LASER 405 nm

    Filter 7 (450/50) : BD horizon V450, Pacific blue, alexa fluor 405, dapi
    Filter 8 (510/50) : AmCyan, SYTOX Blue, BD horizon V500, Pacific orange,

    Posted in 10 parameters analyzer

    CELIGO-cytometer imaging system

    
    Partner lab price : 21.78 €/hour
    Public and private lab price: 59.44 €/hour
    Brief description:
    The celigo is a benchtop microwell plate based, brightfield and fluorescence system.the system provide high speed fully automated imaging and quantification of cells and spheroids.Propriatery optics enable imaging from the center to the edge of the wells by a constant illumination.
    1-LED for brightfield
    3-LED for fluorescence
    blue filter (ex377/50 em470/20) :Dapi/hoechst
    green filter (ex483/32 em536/40) :fitc,gfp,AF488
    red filter(ex531/40 em629/53) RPE,PI ,Texas red, AF568
    -lentille F-theta
    galvanometric mirrors
    CCD camera (2024x2024)
    1.24.8 µM/pixel
    L2 lab

    Posted in 4 parameters analyzer

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