MRI-ISEM

Facility MRI-ISEM has been open since 2010 and is on the ground floor of Evolutionary Sciences Institute on Triolet campus of the University of Montpellier. Facility is accessible 24h/24h 7d/7d for site staff and welcomes external users Campus from 9:00 to 18:00 on weekdays.

PERSONNEL

Facility Manager
MRI-ISEM

Equipments



Our services

The X-ray microtomography platform houses an ex-vivo X-ray microtomograph (EasyTom 150)  and an associated imaging workstation.

 X-Ray microtomography makes it possible to study living and fossil organisms in a non-destructive manner.

The ISEM facilities are housed at the ISEM (ground floor of building #22, campus Triolet of the University of Montpellier).

machine

 

 

 

How to find us?

ISEM (Institut des sciences de l'evolution - Montpellier)
Université de Montpellier - campus triolet

Bâtiment 22, rez-de-chaussée côté route de Mende, Bureau 54
place Eugène Bataillon
34095 Montpellier cedex 05
France

Téléphone : +33 (0) 4 67 14 32 60

GPS : Latitude = 43.63225 ; Longitude = 3.86700

Posted in MRI-ISEM

MRI-PHIV LaGaillarde

Facility imaging MRI-PHIV La Gaillarde has been open since 2006. It is located since 2013 at the 1st floor of the Institute of Plant Sciences in Montpellier (IPSiM), campus SUPAGRO - INRA La Gaillarde, 2 place Viala. Facility is available 7/7 for laboratory staff and hosts IPSiM external users from 8:00 to 19:00 on weekdays.

PERSONNEL

Facility Manager
MRI-PHIV
La Gaillarde

Equipments

Confocal microscopy:


How to find us?

Institut ibip, laboratoire bpmp
umr5004

Bâtiment 7, 2ème étage porte 229
2 place Pierre Viala
34060 Montpellier

Téléphone : (33) (0)4 99 61 26 08

GPS : Latitude = 43.61792; Longitude = 3.85714

Posted in MRI-PHIV-LaGaillarde

MRI-PHIV Lavalette

Facility imaging MRI-PHIV-Lavalette opened in 2003 and located at CIRAD, Agropolis campus.
Facility is available for site personnel and welcomes external users Campus Monday to Friday from 7 am to 19.45.

PERSONNEL

Facility Manager by interim
MRI-PHIV
Lavalette
Manager Facility by interim
Imaging /
Image analysis
Laser Microdissection /
Imaging

Equipments

Widefield microscopy:

Laser microdissection:


How to find us?

CIRAD agropolis

Bâtiment 2, rez-de-chaussée bureaux 7, 8 et 44
avenue d'Agropolis
34398 Montpellier Cedex 05

Téléphone : +33 4 67 61 75 30

GPS : Latitude = 43.65062 ; Longitude = 3.86997

Posted in MRI-PHIV-Lavalette

MRI-PHIV-DIADE

The MRI-PHIV-DIADE imaging facility has been open since January 2023 and is located at IRD (Agropolis campus), in the Capmeditrop building (follow the signage from the reception).
The platform is accessible for IRD staff and welcomes external users from Monday to Friday from 9 a.m. to 6 p.m.

PERSONNEL

Facility Manager
MRI-PHIV-DIADE

Equipments

Microscopie confocale:

Stereomicroscopy :

How to find us?

IRD UMR_D232

911 Av. Agropolis
34293 Montpellier

Phone : 04 67 41 61 00

Posted in MRI-PHIV-DIADE

Multi-photon Zeiss LSM 7MP OPO


Partner lab price : 15.66 €/hour
Public and private lab price: 55.41 €/hour
Associated technologies:
  • 2D imaging
  • 3D imaging
  • SHG / THG
  • In vivo imaging

    • Brief description:
    Multi-photon microscope on upright stand, ideal for 3D acquisitions on live samples, in vivo on small animals, thick cleared or not cleared samples.

    Fluorescence
    Label-free imaging: SHG, THG, CARS
    Multi-dimensional acquisition

    Acquisition software: Zen Black 2012
    Objectives:
    10X EC Plan-Neofluar 0.3 NA
    20X Plan Apochromat 1.0 NA Water
    20X Plan Neofluar 1.0 NA Clr Korr RI = 1.45 +/- 0.03 (on request)
    40X Plan Apochromat 1.0 NA Water
    Detection:
    2 PMTs NDD
    2 BiG

    Spectral separation of fluorochromes: several available configurations, from blue to far red

    - - -
    Environmental control (T°)
    - - -
    To locate the sample with eyepieces:
    Excitation source: HXP120V lamp
    Filter cubes: DAPI / GFP / Rhod

    - - -
    Laser sources:
    - Chameleon Ultra II: 680 to 1080 nm
    - Chameleon Compact OPO (optical parametric oscillator): 1000 to 1300 nm
    - - -
    Types of acquisition:
    - multi-modal imaging: XYZ, time lapse, multichannel, mosaïc, multi-positions
    - imaging on small animal
    - fluorescence
    - SHG: second harmonic generation
    - THG: third harmonic generation
    - CARS: Coherent Anti-Stokes Raman Scattering

    Posted in Multiphotonic microscopy

    Nanozoomer 2 Hamamatsu

    
    Partner lab price : 17.47 €/hour
    Public and private lab price: 82.24 €/hour
    Associated technologies:
  • 2D imaging

    • Brief description:
    high performance imaging systems, ATM, dedicated to immuno-histological marking (color image). essential tools for toxicity screening studies and broadband and storage of digital slides after staining or immunostaining.

    Led by NDP.scan
    Objectives :
    20X
    40X
    Detector:
    camera
    Scan type:
    automatic -Acquisition (210 slices)
    -coloring Histology (color Images)
    -General Secretariat service for digitizing histological slides brightfield.

    Posted in Automated imaging

    NOVOCYTE ACEA 1 (yellow)

    
    Partner lab price : 21.78 €/hour
    Public and private lab price: 59.44 €/hour
    Brief description:
    Flow cytometry cell analyzer - ACEA
    3 lasers, 2 physical parameters and 13 fluorescences
    Plate and tubes loader
    Controlled by the NovoExpress software (ACEA)
    Blue laser excitation- 488nm :
    filter 530/30 BP: FITC, alexa Fluor 488, GFP, CFSE, AF530, Sytox Green
    filter 586/20 BP : YFP
    filter 615/20 BP : IP
    filter 660/20 BP : PercP, 7AAD
    filter 695/40 BP : PerCP-Cy5.5
    Red laser excitation 640 nm:
    filter 660/20 BP : APC, A647
    filter 695/40 BP : AF680, AF700
    filter 780/60BP : APC-Cy7, APC-H7, eF780
    Yellow laser excitation 561 nm:
    filter 586/20 BP : PE, dTomato,DsRed
    filter 615/20 BP : PE-Texas Red, mCherry, dTomato
    filter 660/20 BP : PE-Cy5, mPlum, crimson
    filter 695/40 BP : PE-Cy5.5
    filter 780/60BP : PE-Cy7
    Acquisition of 30 000 events/second

    Intracellular or cell-surface staining, fixed or alive cells

    L2 laboratory completely equiped : hood, centrifuge, pipets
    Ideal operating temperatures between 19 and 26°C

    Posted in 15 parameters analyzer

    NOVOCYTE ACEA 2 (violet)

    
    Partner lab price : 21.78 €/hour
    Public and private lab price: 59.44 €/hour
    Brief description:
    Flow cytometry cell analyzer - ACEA
    3 lasers, 2 physical parameters and 13 fluorescences
    Plate and tubes loader
    Controlled by the NovoExpress software (ACEA)
    Blue laser excitation- 488nm :
    filter 530/30 BP: FITC, Alexa Fluor 488, GFP, CFSE, Sytox Green, FAM-Azide, YFP, BB515
    filter 572/28 BP : PE, (dTomato)
    filter 615/20 BP : PE-TexasRed, PI, (mcherry), PE-CF594
    filter 675/30 BP : PerCp, PerCp-cy5.5, PerCp-eF710, 7AAD, PE-Cy5, PE-cy5.5
    filter 780/60 BP : PE-Cy7
    Red laser excitation 640 nm:
    filter 675/30 BP : APC, A647, Crimson, Cy5, Sytox Red, eF660
    filter 780/60BP : APC-Cy7, APC-H7, APC-AF750, APC-eF780
    Violet laser excitation 405 nm:
    filter 445/45 BP : BD Horizon V450, Pacific Blue, AF405, BV421, Sytox blue, Dapi, BFP
    filter 530/30 BP : BD Horizon V500, AmCyan, BV510
    filter 572/28 BP : Pacific Orange, BV570
    filter 615/20 BP : BV605
    filter 675/30 BP : BV650
    filter 780/60 BP : BV785, (BV750, BV711)

    Acquisition of 30 000 events/second

    Intracellular or cell-surface staining, fixed or alive cells

    L2 laboratory completely equiped : hood, centrifuge, pipets
    Ideal operating temperatures between 19 and 26°C

    Posted in 15 parameters analyzer

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