ImageXpress Pico


Partner lab price : 17.47 €/hour
Public and private lab price: 82.24 €/hour

Brief description:
Automated epifluorescent widefield microscope. Ideal for medium throughput image acquisition and analysis with a large number of samples (cellules, sphéroïdes...) fixed or live and in microplate format. Color and time-lapse imaging possible.
Piloted by CellReporterXpress
Objectives Leica:
4X/NA 0.13 FLUOTAR
10X/NA 0.32 FLUOTAR
20x/NA 0.40 FLUOTAR
40x/NA 0.60 FLUOTAR
Excitation Source:
6 LEDs : Cy5, TRITC, FITC, DAPI, Texas Red, CFP, white light (and RGB)

Filter cubes:
DAPI
FITC
TRITC
Cy5

Can read any format of microplate (6 à 384 wells) and conventional microscopy slides (x4)
Detector:
CMOS camera 5Mpx 12bits
(2000 x 2000 pixels image)

Environmental control: temperature, humidity and CO²
Applications:
-multidimensional automated acquisition: YXZ, multi-channels, multi-fileds, multi-wells, multi-plates, timelaps
-easy image analysis (on-the-fly): 25 preconfigured protocols: Cell counting, Multi-channel Cell scoring, Translocation, Neurite tracing...
-On-the-fly stitching and deconvolution (2D)
-hardware and image autofocus

Opera Phenix


Partner lab price : 37.04 €/hour
Public and private lab price: 101.89 €/hour
Associated technologies:
  • High Content Screening
  • Image analysis

    • Brief description:
    Automated epifluorescent widefield and confocal (spinning disk) microscope with 2 cameras. Ideal for high throughput measure and analysis of cellular content with a large number of samples (cellules, sphéroïdes...) fixed or live and in microplate format.
    Piloted by Harmony software
    Objectives:
    5X air 0.16NA Plan Apochromat
    10X air 0.3NA EC Plan Neofluar
    20X air O.4NA LD Plan Neofluar
    20X air O.8NA Plan Apochromat
    63X eau 1.15NA LD C-Apochromat

    Twister III robotic arm to automatically charge the microplates
    Excitation Source:
    4 Lasers : 405 nm, 488 nm, 561 nm et 640 nm

    Emission filters:
    435-550 nm, -435-480 nm, 500-550 nm, 570-630 nm et 650-760 nm

    Can read any format of microplate (6 à 1536 wells)
    Detector:
    2 cameras sCMOS 4.7Mpx 16bits
    (2160 x 2160 pixels, 6.5µm pixel size)

    Environmental control: temperature and CO²
    Applications:
    -multidimensional automated acquisition: YXZ, multi-channels, multi-fileds, multi-wells, multi-plates, timelaps
    -on-the-fly image analysis: personnalized protocol based on different building blocks pipeline
    -Preciscan XYZ: detection of rare objects or events at low magnification and automated ReScan at higher magnification
    -Analysis and classification of objects by Machine Learning

    Spinning disk Dragonfly

    
    Partner lab price : 17.47 €/hour
    Public and private lab price: 82.24 €/hour
    Associated technologies:
  • 3D imaging
  • Videomicroscopy

    • Brief description:
    Nikon inverted micorsopce coupled to the Andor Dragonfly spinning disk dedicated to 3D confocal imaging of live samples or fixed samples. This microscope is also equiped 2 cameras, for simultaneous 2 color live imaging.

    Controlled by the Andor fusion software.
    Objectives:
    10X Plan Apo lambda 0.45 NA 4mm WD dry
    20X Plan Apo lambda 0.75 NA 1mm WD oil
    40X Plan Fluor 1.3 NA 0.2mm WD oil
    40X Apo 1.15 NA 0.6mm WD water
    60X Plan Apo lambda 1.4 NA 0.13mm WD oil
    100X Plan Apo lambda 1.45 NA 0.13mm WD oil

    Detector:
    2 camaras EMCCD iXon888 Life Andor
    (1024*1024 pixels, 13µm pixel size, 2x lens if needed, QEmax 95%)

    Environmental control: temperature and CO2
    Illumination sources:
    1 laser bench for confocal mode:
    405nm (100mW), 488nm (150mW), 561nm (100mW) et 637nm (140mW)

    Spectral configuration:
    2 dichroïcs in the head unit: either 100% to one camera or DAPI/GFP for vertical camera and mCherry/Cy5 for other camera with 565 dichroic mirror
    6 emission filters in the external wheel:
    DAPI (450/50), GFP (525/50), mCherry (620/60), Cy5 (700/775), GFP/mCher
    Type of acquisition:
    multi-dimensional acquisition in bright field or DIC transmission and confocal in fluorescence: XYZ (Z piezo of range 300um), multi-channels, multi-position, tile-scans, multi-wells, timelapse
    hardware autofocus (PFS)

    Compatible sample holders:
    35mm dish with #1.5 glass bottom, glass slide with #1.5 glass coverslip or glass-bottom multi-well plate