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Partner lab price : 15.66 €/h
Public and private lab price55.41 €/h
Associated technologies:

Brief description:
Multi-photon microscope on upright stand, ideal for 3D acquisitions on live samples, in vivo on small animals, thick cleared or not cleared samples.

Fluorescence
Label-free imaging: SHG, THG, CARS
Multi-dimensional acquisition

Acquisition software: Zen Black 2012
Objectives:
10X EC Plan-Neofluar 0.3 NA
20X Plan Apochromat 1.0 NA Water
20X Plan Neofluar 1.0 NA Clr Korr RI = 1.45 +/- 0.03 (on request)
40X Plan Apochromat 1.0 NA Water
Detection:
2 PMTs NDD
2 BiG

Spectral separation of fluorochromes: several available configurations, from blue to far red

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Environmental control (T°)
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To locate the sample with eyepieces:
Excitation source: HXP120V lamp
Filter cubes: DAPI / GFP / Rhod

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Laser sources:
- Chameleon Ultra II: 680 to 1080 nm
- Chameleon Compact OPO (optical parametric oscillator): 1000 to 1300 nm
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Types of acquisition:
- multi-modal imaging: XYZ, time lapse, multichannel, mosaïc, multi-positions
- imaging on small animal
- fluorescence
- SHG: second harmonic generation
- THG: third harmonic generation
- CARS: Coherent Anti-Stokes Raman Scattering