Spinning disk Nikon Ti Inscoper CSU-X1

Partner lab price : 15.66 €/h
Public and private lab price : 55.41 €/h
Associated technologies:
Brief description:
Nikon inverted TiE micorsopce coupled to a spinning Yokogawa CSU-X1 unit dedicated to 3D confocal imaging of live samples. All the peripheriques are controled by the Inscoper electronic box, that increases the acquisition speed (triggering).

Controlled by the Inscoper software
Objectives:
10X Plan 0.25 NA dry
20X Plan Apo lambda 0.75 NA dry
40X Plan Fluor 1.3 NA oil
60X Plan Apo lambda 1.4 NA oil
100X Plan Apo lambda 1.45 NA oil

Detector:
1 sCMOS back-illuminated PRime95B Photometrics
(1200*1200 pixels, 11µm pixel size)

Environmental control: temperature and CO2
Illumination sources:
1 laser bench Oxxius:
405nm (50mW), 488nm (100mW), 561nm (100mW) and 638nm (100mW)

Spectral configuration:
3 dichroïcs in the head unit: MONO (488), DUAL (488-561) and QUAD (405-488-561-640)
6 emission filters in the external wheel:
DAPI(447/60), GFP(525/30), RFP(607/36), Cy5(685/40), GFP/Cherry(512-23/630-90), DAPI/GFP/Cherry/Cy5 (440-40/521-21/607-34/700-45)
Type of acquisition:
multi-dimensional acquisition in bright field or DIC transmission and confocal in fluorescence: XYZ, multi-channels, multi-position, mosaïcs, multi-wells, timelapse
hardware autofocus
custom-made protocol thank to Inscoper software
Compatible sample carriers:
35mm dish with #1.5 glass bottom, glass slide with #1.5 glass coverslip or glass-bottom multi-well plate