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Partner lab price : 15.66 €/h
Public and private lab price55.41 €/h
Associated technologies:

Brief description:
Inverted Nikon TiE wide-field microscope for timelapse in 2D or 3D in fluorescence or transmitted light mode (BF, DIC). Trigger option for fast imaging.

Controled by NIS Elements.
Objectives:
10X Plan Apochromat 0.45NA air
20X Plan Apochromat 0.75NA air
40X Plan Fluor 1.3NA oil
60X Plan Apochromat 1.4NA oil
100X Plan Apochromat 1.40NA oil

Detectors:
1 camera sCMOS back-illuminated Prime95B Photometrics
(1200*1200, 11µm pixel size)
Incubation:
temperature and CO2 control

Type of acquisition :
-widefield epifluorescent microscopy
-transmitted light microscope in bright field or DIC mode.

PFS module for control of the z drift during timelapse.

Trigger option for fast multicolor imaging.
Illumination source:
Lumencor with 7 LED and excitation filters for:
DAPI, CFP, GFP, YFP, Cy3, Cherry, Cy5

Emission filters:
DAPI, CFP, GFP, YFP, Cy3, Cherry, Cy5
Compatible sample holders:
35mm dish with #1.5 glass bottom, glass slide with #1.5 glass coverslip or glass-bottom multi-well plate