MuViSPIM

Partner lab price : 15.66 €/h
Public and private lab price : 55.41 €/h
Technologies associées :

Brève description :
Luxendo Light Sheet microscope for 3D live imaging of small organisms (such as embryos). The set-up has 2 illumination arms with 4 laser lines (488, 515, 561 and 642nm) and 2 emission arms with 2 emission objectives (33.3X final magnification).

Controlled by Luxendo software
Objectives:
10X Plan FLUOR 0.3NA Water Nikon *2
20X XLUMPlanFL N 1.0NA Water Olympus *2

Detectors:
2 sCMOS Hamamatsu Orca Flash 4 V2 cameras
(2048*2048, 6.5µm pixel size)

Incubation:
temperature between 10 and 40°C and CO2 control
Acquisition modes:
Light sheet imaging in multi-channels mode (widefield or confocal), in multi-Z stacks, in multi-timepoints, in multi-angles with the sample rotation
Z-piezo stage with 20nm resolution, XY stage with 100nm resolution and sample rotation in 360° angle
Illumination sources:
laser 488nm
laser 515nm
laser 561nm
laser 642nm
Light sheet generated with a digital light scanner

Spectral selection:
5 emission filters in a filter wheel
GFP (488 LP)
GFP en combinaison (525/50 BP)
YFP (535/30 BP)
Cherry (561 LP)
FarRed (642 LP)
Sample carriers:
the sample is prepared in a glass capillary either embedded in a gel or loaded in a well formed in the gel. This capillary is charged in the sample carrier on the stage.
Each project done on this MuViSPIM needs specific development for the sample preparation