If you use several fluorochromes (for example A and B) in a single experiment, the emission spectra may overlapped. Thus, it is necessary to compensate the signals, that is to say to eliminate the fluorescence provided by the A fluorochrome in the channel where you read the B fluorescence.

To well set up the compensations, you need mono-staining of each used fluorochrome in the experiment.
Please, contact the manager of the facility to optimize your fluorochrome choices regarding the cytometer installed on the facility.