ARIA IIu Becton Dickinson

Partner lab price : 48.99 €/h
Public and private lab price : 131.03 €/h
Brief description:
Analyzer and cell sorter by flow cytometry - Becton Dickinson
4 lasers, 2 physical parameters and 17 fluorescences

Controlled by the FACSDiVa software (Becton Dickinson)
Blue laser excitation- 488nm :
Filter 1 (505LP - 530/30BP) : FITC, AF488, GFP, CFSE, BB515
Filter 2 (550LP - 575/25BP) : PE
Filter 3 (600LP - 610/20BP) : mCherry, PI, PE-TexasRed, PE-CF594
Filter 4 (635LP - 660/20BP) : PE-Cy5, 7-AAD
Filter 5 (685LP - 710/50BP) : PerCP, PerCP-Cy5.5, PerCP-eF710
Filter 6 (755LP - 780/60BP) : PE-Cy7
Violet laser excitation - 405nm :
Filter 10 (450/50BP) : BD Horizon V450, Pacific Blue, AF405, BV421
Filter 11 (505LP - 525/50BP) : BD Horizon V500, Pacific Orange, AmCyan, Qdot525, BV510
Filter 12 (595LP - 610/20BP) : Qdot605, BV605, eF605NC, eF625NC
Filter 13 (630LP - 670/30BP) : Qdot655, BV650, eF650NC
Filter 14 (685LP - 710/50BP) : Qdot700, BV711
Filter 15 (750LP - 780/60BP) : Qdot800, BV785
Red laser excitation - 633nm :
Filter 7 (660/20BP) : APC, AF647
Filter 8 (690LP - 730/45BP) : AF700, APC-R700
Filter 9 (755LP - 780/60BP) : APC-Cy7, eFluor780, AAF

UV laser Excitation - 355nm :
Filter 16 (450/50BP) : DAPI, Hoechst, Hoechst 33258, Hoechst Blue, Indo-1 (violet)
Filter 17 (505LP - 530/30BP) : Hoechst Red, Indo-1 (blue)
High speed cell sorting (up to 25000 events/sec) on 2 or 4 ways, ACDU (automatic cell deposition unit) for plates (6, 12, 24, 96 or 384 wells) or slides.
Sterile sorts with possibility to expand the sorted cells in culture
Intracellular or cell-surface staining, fixed or alive cells.

L2 laboratory completely equiped : hood (subject to booking), centrifuge, pipets
Ideal operating temperatures between 19 and 26°C