LSR FORTESSA Becton Dickinson

Partner lab price : 25.68 €/h
Public and private lab price : 50.75 €/h
Brief description:
Flow cytometry cell analyzer - Becton Dickinson
4 lasers, 2 physical parameters and 16 fluorescences

Controlled by the FACSDiVa software (Becton Dickinson)
Blue laser excitation 488nm (50mW) :
Filter 1 (530/30BP) : FITC, AF488, GFP, CFSE, BB515
Filter 2 (685LP - 710/50BP) : PerCP, PerCPCy5.5, PerCP-eF710

Yellow-Green laser excitation 561nm (50mW) :
Filter 3 (585/15BP) : PE, DsRed
Filter 4 (600LP - 610/20BP) : mCherry, PI, PE-TexasRed, PE-CF594
Filter 5 (635LP - 670/30BP) : PE-Cy5, 7-AAD
Filter 6 (685LP - 710/50BP) : PE-Cy5.5
Filter 7 (750LP - 780/60
Violet laser excitation 400nm (50mW) :
Filter 11 (450/50BP) : BD Horizon V450, Pacific Blue, AF405, DAPI, BV421
Filter 12 (475LP - 525/50BP) : BD Horizon V500, Pacific Orange, AmCyan, Qdot525, BV510
Filter 13 (595LP - 610/20BP) : Qdot605, BV605, eF605NC, eF625NC
Filter 14 (630LP - 660/20BP) : Qdot655, BV650, eF650NC
Filter 15 (685LP - 710/20BP) : Qdot700, BV711
Filter 16 (750LP - 780/60BP) : BV786
Red laser excitation 633nm (40mW) :
Filter 8 (670/14BP) : APC, AF647
Filter 9 (690LP - 730/45BP) : AF700, APC-R700
Filter 10 (750LP - 780/60BP) : APC-Cy7, APC-H7, APC-AF750, APC-eF780
High speed acquisition : 20 000 events/sec
High sensitivity photomultiplier (PMT)

Intracellular or cell-surface staining, fixed or alive cells

L2 laboratory completely equiped : hood, centrifuge, pipets
Ideal operating temperatures between 19 and 26°C